RBIO-05. QUANTIFICATION OF TSPO EXPRESSION AND TSPO RADIOLIGAND UPTAKE IN PET IN HUMAN AND MURINE GLIOBLASTOMA ON CELLULAR LEVEL
نویسندگان
چکیده
Abstract OBJECTIVE Translocator protein (TSPO) PET imaging is used in different neurological diseases and emerges as new tool for the diagnosis therapy planning of glioma patients. Various signal sources TSPO-PET signal, however, hamper image interpretation biological understanding. Here we assess TSPO expression ligand uptake a murine model human tissue derived from METHODS Mice with implanted GFP+ glioblastoma (n=20) or after sham implantation (n=14) were injected tracer [18F]GE-180 received imaging. Tumors harvested processed to single cell suspension. Subsequently, tumors either stained TSPO, CD11b, ACSA2, GFAP analyzed by flowcytometry else magnetic sorting was performed isolate tumor CD11b+ cells that measured gamma counter. Multiple regression determine contributions GFP, ACSA2 positive total uptake. In PET-scans patients, regions GE-180 defined. Tumor samples (n=12) these predefined areas collected during surgery FACS described above. RESULTS The mice increased compared tumor-free brain (SUVRGBM: 2.06 / SUVRSHAM: 0.92). detected cells, microglia/macrophages astrocytes. showed higher per when (+31%). contribution whole high (β=0.41, P=0.018) (β=0.35, P=0.042), lowest astrocytes (β=0.28, P=0.011). Patients high-grade indicated GFAP+ correlated signal. CONCLUSION experimental orthotopic glioblastoma, could detect at cellular resolution level. can provide information about tumor-associated myeloid cells.
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ژورنال
عنوان ژورنال: Neuro-oncology
سال: 2022
ISSN: ['1523-5866', '1522-8517']
DOI: https://doi.org/10.1093/neuonc/noac209.957